LSR
II(BD)
Bench-top Analyser
- Four laser
355nm UV
405nm violet
488nm Sapphire
633 HeNe
- 19 parameter
-
cytometer for 17
(2, 6, 6, 3) colour analysis
| Performance (Top) | |
| Fluorescence Sensitivity | FITC-A : 80 molecules of equivalent
soluable fluorophore PE-A : 30 molecules of equivalent soluable fluorophore |
| Fluorescence Resolution | Coefficient of variation in PI-Area of < 3%, full G0/G1 peak for propidium iodide–stained chicken erythrocyte nuclei |
| Forward
and Side Scatter Sensitivity |
Sensitivity enables the separation of fixed platelets from noise, identification of bacteria and 0.5 micron beads |
| Forward
and Side Scatter Resolution |
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes |
| Acquistion rate | >20,000 events/second(2x107 cells/ml @ 60ul/min) |
| Excitation Optics (Top) | |
| Optical Platform | Fixed optical assembly on cuvette flow cell |
| Lasers | 20mW 355nm, Lightwave solid state 20mW 488nm, Sapphire argon-ion 20mW 635nm, Uniphase HeNe |
| Emission Optics (Top) | |
| Optical Coupling | Quartz cuvette is coupled to emission
lens by refractive index matching optical gel for optimum collection
efficiency Laser steering delivers spacially separated beams to flow cell Fixed fiber apertures (125um separation) |
| Fluorescence detectors | High performance, high dynamic range photomultipliers and red-sensitive photomultipliers with filters |
405nm Fluorescence Detectors and Filters |
450/50nm PB/BV 421/Alexa 405/V450 525/5nm BV 470/Qd 525/V500 /KO 560/20nm PO/Qd 565 585/15nm BV 570/Qd 585 605/12nm BV 605/Qd 605 655/8nm BV 650/Qd 655 |
488nm Fluorescence
Detectors and Filters |
525/30nm
FITC/Alexa-488/GFP/CFSE 575/26nm PE/DsRED 610/20nm PE-TR/ PI 670/14nm PerCP/PE-Cy5/7AAD 695/40nm PerCP-Cy5.5 780/60nm PE-Cy7 |
635nm Fluorescence
Detectors and Filters |
660/20nm
APC/Alexa-647 730/45nm Alexa-700 780/60nm APC-Cy7/APC-Alexa-750 |
355nm Fluorescence
Detectors and Filters |
450/40nm
Indo-1(bound)/Alexa-405 530/30nm DAPI/Hoechst/Indo-1(free) |
| Fluidics (Top) | |
| General Operation | Front key panel control provides three modes: RUN, STNDBY, and PRIME; automatic standby mode conserves sheath fluid by stopping sheath flow when no sample tube is installed |
| Fluid Reservoirs | Easily accessible 20L capacity sheath and waste containers ; level detectors automatically indicate low levels of sheath or high levels of waste |
| Sample Flow Rates | Variable flow rates of 120-30 µL/min(HI),
70-17.5 µL/min(MED), and 24-6 µL/min(LOW). Regulated pressure difference between sheath and sample |
| Quartz Cuvette | Internal cross-section is rectangular 430 x 180-µm; external surfaces are anti-reflection coated for maximum transmission of laser light |
| Sample Concentration | Single-cell suspension of 105 to 2 x 107 particles/mL recommended range |
| Signal Processing (Top) | |
| Workstation Resolution | 262,144 linear channels displayed in a 5 log decade display |
| Data Acquisition Channels | 13 parameters- 11 colour and 2 scatter |
| Fluorescence Compensation Networks | Inter- and intra-beam software programmable resolution of 0.01% |
| Pulse Processing | Width and Area measurements for discriminating doublets; available for all fluorescence parameters; Ratio measurements for intra-laser parameters |
| Time | Time available correlated to any parameter for kinetic experiments or other applications. |
| Data Management System (Top) | |
| Workstation | PC |
| Central Processing Unit (CPU) | Power PC Reduced Instruction Set Computer (RISC) CPU |
| Memory | 2GB RAM |
| Level 2 Cache | |
| Data Storage | |
| Networking | |
| CD ROM | DVD/CD-ROM read/write combo drive |
| Monitor | 2x19-inch MultiSync Monitors |
| Data File Structure | Flow Cytometry Standard (FCS) 2.0 and 3.0 ASCII results file for data export |
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