Canto II (BD)
Bench-top Analyser
- triple laser
405nm violet
488nm Sapphire
633 HeNe
- 10 parameter
-
cytometer for 8
(2, 4, 2) colour analysis
| Performance (Top) | |
| Fluorescence Sensitivity | FITC-A : <100 molecules of equivalent soluable fluorophore PE-A : <50 molecules of equivalent soluable fluorophore |
| Fluorescence Resolution | < 3% for PI labelled chick erythrocyte nuclei |
| Forward and Side Scatter Sensitivity |
Sensitivity enables the separation of fixed platelets from noise, identification of bacteria and 0.5 micron beads |
| Forward and Side Scatter Resolution |
FSC 1.0µm SSC 0.5µm |
| Acquistion rate | max 10,000 events/second(1x107 cells/ml @ 120ul/min) |
| Excitation Optics (Top) | |
| Optical Platform | Fixed optical assembly on cuvette flow cell |
Lasers Air cooled |
30mW 407nm 20mW 488nm, 17mW 635nm, |
| Emission Optics (Top) | |
| Optical Coupling | Quartz cuvette is coupled to emission
lens by refractive index matching optical gel for optimum collection
efficiency Laser steering delivers spacially separated beams to flow cell Fixed fiber apertures (125um separation) |
| Fluorescence detectors | High performance, high dynamic range photomultipliers and red-sensitive photomultipliers with filters |
405nm Fluorescence Detectors and Filters |
450/50nm PB/BV 421/Alexa 405/V450 525/5nm BV 470/Qd 525/V500 /KO |
488nm Fluorescence
Detectors and Filters |
525/30nm
FITC/Alexa-488/GFP/CFSE 585/42nm PE/DsRED 670LPnm PerCP/PE-Cy5/7AAD 780/60nm PE-Cy7 |
635nm Fluorescence
Detectors and Filters |
661/20nm
APC/Alexa-647 780/60nm APC-Cy7/APC-Alexa-750 |
| Fluidics (Top) | |
| General Operation | Integrated fluidics cart and compressor with onboard housekeeping solutions for automated startup, shutdown, and cleaning cycles |
| Fluid Reservoirs | Easily accessible 20L capacity sheath and waste containers ; level detectors automatically indicate low levels of sheath or high levels of waste |
| Sample Flow Rates | Flow rates of 120µL/min(HI),
60µL/min(MED), and 10µL/min(LOW). Regulated pressure difference between sheath and sample |
| Quartz Cuvette | Internal cross-section is rectangular 430 x 180-µm; external surfaces are anti-reflection coated for maximum transmission of laser light |
| Sample Concentration | Single-cell suspension of 105 to 2 x 107 particles/mL recommended range |
| Signal Processing (Top) | |
| Workstation Resolution | 262,144 linear channels displayed in a 5 log decade display(18bit) |
| Data Acquisition Channels | 10 parameters- 8 colour and 2 scatter |
| Fluorescence Compensation Networks | Inter- and intra-beam software programmable resolution of 0.01% |
| Pulse Processing | Width, Height and Area measurements for discriminating doublets; available for all fluorescence parameters; Ratio measurements for intra-laser parameters |
| Time | Time available correlated to any parameter for kinetic experiments or other applications. |
| Data Management System (Top) | |
| Workstation | PC |
| Central Processing Unit (CPU) | Intel Core 2 Duo E6850
Dual-Core3 3.00-GHz, 4MB shared L2 cache, 1333 MHz FSB |
| Memory | 2GB RAM |
| Software | BD FACSDiva 6.1.3 |
| Data Storage | |
| Networking | |
| CD ROM | DVD/CD-ROM read/write combo drive |
| Monitor | 2x19-inch MultiSync Monitors |
| Data File Structure | Flow Cytometry Standard (FCS) 2.0 and 3.0 ASCII results file for data export |
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